| City of Cape Town | WC Western Cape | — | 25 | 269 | Table Mountain National Park |
| City of Tshwane | GP Gauteng | — | 22 | 274 | Dinokeng Game Reserve |
| eThekwini | KZN KwaZulu-Natal | — | 22 | 3 | Kenneth Stainbank Nature Reserve |
| Mogale City | GP Gauteng | West Rand | 16 | 1 | Bolt's Farm |
| Bushbuckridge | MP Mpumalanga | Ehlanzeni | 15 | 3 | Sabiepark |
| Bela-Bela | LP Limpopo | Waterberg | 12 | 48 | Sondela Nature Reserve |
| Overstrand | WC Western Cape | Overberg | 12 | 2 | Phillipskop Mountain Reserve |
| Big Five Hlabisa | KZN KwaZulu-Natal | Umkhanyakude | 11 | 15 273 | iSimangaliso Wetland Park |
| Buffalo City | EC Eastern Cape | — | 11 | 6 | Umtiza Nature Reserve |
| Saldanha Bay | WC Western Cape | West Coast | 11 | — | Cape Columbine Nature Reserve |
| City of Johannesburg | GP Gauteng | — | 10 | 4 | Modderfontein Reserve |
| Madibeng | NW North West | Bojanala | 10 | 0 | Gondolin Cave |
| Makana | EC Eastern Cape | Cacadu | 10 | 155 | Sam Knott Nature Reserve |
| Nelson Mandela Bay | EC Eastern Cape | — | 10 | 8 | Island Nature Reserve |
| Stellenbosch | WC Western Cape | Cape Winelands | 10 | 1 830 | Boland Mountain Complex |
| Ba-Phalaborwa | LP Limpopo | Mopani | 9 | 20 193 | Kruger National Park |
| Mbombela | MP Mpumalanga | Ehlanzeni | 9 | 80 | Mthethomusha Game Reserve |
| Modimolle/Mookgophong | LP Limpopo | Waterberg | 9 | — | Bateleur Nature Reserve & Stamvrug Hiking Trails |
| Thabazimbi | LP Limpopo | Waterberg | 9 | 235 | Atherstone Nature Reserve |
| Ventersdorp/Tlokwe | NW North West | Dr Kenneth Kaunda | 9 | 301 | Vredefort Dome core area |
| Bitou | WC Western Cape | Eden | 8 | 1 210 | Garden Route National Park |
| Knysna | WC Western Cape | Eden | 8 | 0 | Pledge Nature Reserve |
| Musina | LP Limpopo | Vhembe | 8 | 428 | Matshakatini Nature Reserve |
| Umhlabuyalingana | KZN KwaZulu-Natal | Umkhanyakude | 8 | 1 795 | Turtle Beaches / Coral Reefs of Tongaland |
| Cederberg | WC Western Cape | West Coast | 7 | 1 548 | Cederberg |
| Impendle | KZN KwaZulu-Natal | Umgungundlovu | 7 | 2 478 | UKhahlamba / Drakensberg Park |
| Lephalale | LP Limpopo | Waterberg | 7 | 6 540 | Waterberg Biosphere |
| Makhado | LP Limpopo | Vhembe | 7 | 40 | Morning Sun Nature Reserve |
| Maruleng | LP Limpopo | Mopani | 7 | 115 | Thornybush Game Reserve |
| Mogalakwena | LP Limpopo | Waterberg | 7 | 547 | Lapalala Wilderness |
| Moses Kotane | NW North West | Bojanala | 7 | 550 | Pilanesberg National Park |
| Mossel Bay | WC Western Cape | Eden | 7 | — | Bon Game Reserve |
| Ray Nkonyeni | KZN KwaZulu-Natal | Ugu | 7 | — | Lake Eland Game Reserve |
| Thaba Chweu | MP Mpumalanga | Ehlanzeni | 7 | — | Blyde River Canyon Nature Reserve |
| uMngeni | KZN KwaZulu-Natal | Umgungundlovu | 7 | — | Doreen clark nature reserve |
| Drakenstein | WC Western Cape | Cape Winelands | 6 | — | Bontebok Ridge Reserve |
| Hessequa | WC Western Cape | Eden | 6 | — | Gouritz Cluster |
| Swartland | WC Western Cape | West Coast | 6 | 0 | Tienie Versveld Wildflower Reserve |
| Inkosi Langalibalele | KZN KwaZulu-Natal | Uthukela | 5 | 80 | Zingela Safari & River Company |
| Kouga | EC Eastern Cape | Cacadu | 5 | — | Kabeljous Nature Reserve |
| Ndlambe | EC Eastern Cape | Cacadu | 5 | — | Fort D' Acre Reserve |
| Raymond Mhlaba | EC Eastern Cape | Amathole | 5 | — | Double Drift nature reserve |
| uPhongolo | KZN KwaZulu-Natal | Zululand | 5 | 290 | Ithala Game Reserve |
| Bergrivier | WC Western Cape | West Coast | 4 | — | Beaverlac |
| Cape Agulhas | WC Western Cape | Overberg | 4 | 397 | De Hoop Nature Reserve |
| Chief Albert Luthuli | MP Mpumalanga | Gert Sibande | 4 | — | Doornkop Fish and Wildlife Reserve |
| Dihlabeng | FS Free State | Thabo Mofutsanyane | 4 | 116 | Golden Gate Highlands National Park |
| Dr Nkosazana Dlamini Zuma | KZN KwaZulu-Natal | Sisonke | 4 | — | Coleford Nature Reserve |
| Ekurhuleni | GP Gauteng | — | 4 | — | Korsman Conservancy |
| Emakhazeni | MP Mpumalanga | Nkangala | 4 | 5 | Middelpunt Nature Reserve |
| Greater Tzaneen | LP Limpopo | Mopani | 4 | 3 | Modjadji Nature Reserve |
| Kou-Kamma | EC Eastern Cape | Cacadu | 4 | — | Huisklip Local Authority Nature Reserve |
| Maluti a Phofung | FS Free State | Thabo Mofutsanyane | 4 | — | QwaQwa National Park |
| Mangaung | FS Free State | — | 4 | 1 | Free State National Botanical Garden |
| Mtubatuba | KZN KwaZulu-Natal | Umkhanyakude | 4 | 167 | Saint Lucia Park |
| Sundays River Valley | EC Eastern Cape | Cacadu | 4 | 1 780 | Addo Elephant National Park |
| Swellendam | WC Western Cape | Overberg | 4 | 170 | Boosmansbos Wilderness Area |
| Witzenberg | WC Western Cape | Cape Winelands | 4 | — | Ceres Nature Reserve |
| Abaqulusi | KZN KwaZulu-Natal | Zululand | 3 | — | Elsewhere Game Reserve |
| Dawid Kruiper | NC Northern Cape | Z F Mgcawu | 3 | 38 000 | Kgalagadi Transfrontier Park |
| Great Kei | EC Eastern Cape | Amathole | 3 | — | Amathole Marine Protected Area |
| Hantam | NC Northern Cape | Namakwa | 3 | 75 | Oorlogskloof Nature Reserve |
| Jozini | KZN KwaZulu-Natal | Umkhanyakude | 3 | 333 | uMkhuze Game Reserve |
| Langeberg | WC Western Cape | Cape Winelands | 3 | 43 | Drie Kuilen Private Nature Reserve |
| Midvaal | GP Gauteng | Sedibeng | 3 | — | Bloufontein Wildlife Park |
| Mkhambathini | KZN KwaZulu-Natal | Umgungundlovu | 3 | 6 | Mpushini Protected Environment |
| Nama Khoi | NC Northern Cape | Namakwa | 3 | — | Brakputs Private Nature Reserve |
| Phumelela | FS Free State | Thabo Mofutsanyane | 3 | 249 | Sneeuwberg Protected Environment |
| Ramotshere Moiloa | NW North West | Ngaka Modiri Molema | 3 | — | Koos Swart-Privaat Natuurreserwe |
| Rustenburg | NW North West | Bojanala | 3 | — | Kgaswane Nature Reserve Rustenburg |
| Setsoto | FS Free State | Thabo Mofutsanyane | 3 | — | Moolmanshoek Leadership centre |
| Umdoni | KZN KwaZulu-Natal | Ugu | 3 | 22 | Vernon Crookes Nature Reserve |
| uMshwathi | KZN KwaZulu-Natal | Umgungundlovu | 3 | 3 | Cumberland Nature Reserve |